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Anti-HPV18 L1/Major capsid protein L1 Polyclonal Antibody (VK639014)

Anti-HPV18 L1/Major capsid protein L1 Polyclonal Antibody
Anti-HPV18 L1/Major capsid protein L1 Polyclonal Antibody
Price(USD): $
Spec:
  • 50ug
  • 100ug
Number:
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  • Datasheet

Overview
Catalog No.VK639014
Species reactivityHuman papillomavirus type 18
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant HPV18 L1/Major capsid protein L1 (Val82-Gly535).
Target Major capsid protein L1, L1
Purification Purified by antigen affinity column.
Accession P06794
RRID Anti-HPV18 L1/Major capsid protein L1 Polyclonal Antibody (abinScience Cat# VK639014, RRID:AB_3723062)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Major capsid protein L1 is a ~63 kDa protein. Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis.

Note For research use only.
Images
  • Anti-HPV18 L1/Major capsid protein L1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HPV18 L1/Major capsid protein L1 polyclonal antibody at 1ug/mL on recombinant HPV18 L1/Major capsid protein L1 (Catalog No. VK639012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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