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Anti-HR Polyclonal Antibody (HB577014)

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Overview
Catalog No.HB577014
Description
Anti-HR Polyclonal Antibody (HB577014) is a rabbit polyclonal antibody detecting Lysine-specific demethylase hairless; 1.14.11.65; [histone H3]-dimethyl-L-lysine(9) demethylase hairless; HR in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human HR (Arg856-Phe1140).
Target Lysine-specific demethylase hairless; 1.14.11.65; [histone H3]-dimethyl-L-lysine(9) demethylase hairless; HR
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43593, P97609, Q61645
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lysine-specific demethylase hairless (HR) is a ~127 kDa protein. Histone demethylase that specifically demethylates both mono- and dimethylated 'Lys-9' of histone H3. May act as a transcription regulator controlling hair biology (via targeting of collagens), neural activity, and cell cycle.

Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-HR Polyclonal Antibody

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