Anti-HSPA9/Mortalin/GRP75 Polyclonal Antibody (HW569024) is a rabbit polyclonal antibody detecting Mortalin in ELISA, IHC, WB. Suitable for Bovine, Dog, Cricetulus griseus, Chicken, and Human.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Species reactivity
Human, Mouse, Rat
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human HSPA9/Mortalin/GRP75 (Lys52-Asp431).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Stress-70 protein, mitochondrial (HSPA9/Mortalin) is a ~73 kDa protein. Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones. In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins. Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis, interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU.
1. Zhai, P. et al. (2008) The Journal of biological chemistry 283, 26098-106. PMID: 18632665
2. Dores-Silva, PR. et al. (2015) PloS one 10, e0117170. PMID: 25615450
3. Srivastava, S. et al. (2017) The Journal of biological chemistry 292, 18075-18090. PMID: 28848044
4. Moseng, MA. et al. (2019) The journal of physical chemistry. B 123, 3383-3396. PMID: 30933555
8. Sane, S. et al. (2014) Cell death & disease 5, e1118. PMID: 24625977
9. Sane, S. et al. (2016) Cell stress & chaperones 21, 313-26. PMID: 26634371
Note
For research use only.
Images
Western blot
Western blot analysis was performed using anti-Mortalin polyclonal antibody at 1μg/ml on various samples. Lane 1: Hela cell lysate Lane 2: K562 cell lysate Lane 3: A549 cell lysate Lane 4: MCF-7 cell lysate Lane 5: HepG2 cell lysate Lane 6: Mouse liver lysate Lane 7: Mouse brain lysate Lane 8: Mouse kidney lysate Lane 9: Rat liver lysate Lane 10: Rat brain lysate
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