Anti-Human ABCC5 Polyclonal Antibody (HT488014)

Name: Anti-Human ABCC5 Polyclonal Antibody
Brand: abinScience
SKU: HT488014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HT488014

Description

Anti-Human ABCC5 Polyclonal Antibody (HT488014) is a rabbit polyclonal antibody detecting ABCC5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ABCC5 (Ala629-Gly847).

Target

ATP-binding cassette sub-family C member 5, 7.6.2.-, 7.6.2.2, Multi-specific organic anion transporter C, MOAT-C, Multidrug resistance-associated protein 5, SMRP, pABC11, ABCC5, MRP5

Purification

Purified by antigen affinity column.

Accession

O15440

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-binding cassette sub-family C member 5 (ABCC5) is a ~160 kDa protein. ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro). Also acts as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins. Confers resistance to the antiviral agent PMEA. Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated.

1. Jedlitschky, G. et al. (2000) The Journal of biological chemistry 275, 30069-74. PMID: 10893247

2. Wielinga, PR. et al. (2003) The Journal of biological chemistry 278, 17664-71. PMID: 12637526

3. Reid, G. et al. (2003) Molecular pharmacology 63, 1094-103. PMID: 12695538

4. Wielinga, P. et al. (2005) Cancer research 65, 4425-30. PMID: 15899835

5. de Wolf, CJ. et al. (2007) The FEBS journal 274, 439-50. PMID: 17229149

6. Jansen, RS. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 6601-6. PMID: 25964343

7. Jansen, RS. et al. (2015) The Journal of biological chemistry 290, 30429-40. PMID: 26515061

8. Wijnholds, J. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 7476-81. PMID: 10840050

9. Wielinga, PR. et al. (2002) Molecular pharmacology 62, 1321-31. PMID: 12435799

Note

For research use only.

Images

References

Datasheet

Anti-Human ABCC5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ABCC5 Polyclonal Antibody (HT488014)

Overview

Images

References

Datasheet

SDS

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