Anti-Human ACOT8 Polyclonal Antibody (HT441014)

Name: Anti-Human ACOT8 Polyclonal Antibody
Brand: abinScience
SKU: HT441014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT441014

Description

Anti-Human ACOT8 Polyclonal Antibody (HT441014) is a rabbit polyclonal antibody detecting ACOT8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACOT8 (Gly21-Leu319).

Target

Acyl-coenzyme A thioesterase 8, Acyl-CoA thioesterase 8, 3.1.2.1, 3.1.2.11, 3.1.2.2, 3.1.2.3, 3.1.2.5, Choloyl-coenzyme A thioesterase, 3.1.2.27, HIV-Nef-associated acyl-CoA thioesterase, Peroxisomal acyl-CoA thioesterase 2, PTE-2, Peroxisomal acyl-coenzyme A thioester hydrolase 1, PTE-1, Peroxisomal long-chain acyl-CoA thioesterase 1, Thioesterase II, hACTE-III, hACTEIII, hTE, ACOT8, ACTEIII, PTE1

Purification

Purified by antigen affinity column.

Accession

O14734

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Acyl-coenzyme A thioesterase 8 (ACOT8) is a ~35 kDa protein. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs. Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT). Is also able to hydrolyze CoA esters of dicarboxylic acids.

1. Ishizuka, M. et al. (2004) Experimental cell research 297, 127-41. PMID: 15194431

2. Liu, LX. et al. (1997) The Journal of biological chemistry 272, 13779-85. PMID: 9153233

3. Watanabe, H. et al. (1997) Biochemical and biophysical research communications 238, 234-9. PMID: 9299485

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ACOT8 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

References

Datasheet

Anti-Human ACOT8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ACOT8 Polyclonal Antibody (HT441014)

Overview

Images

References

Datasheet

SDS

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