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Anti-Human ACSM3 Polyclonal Antibody (HS928014)

Anti-Human ACSM3 Polyclonal Antibody
Anti-Human ACSM3 Polyclonal Antibody
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Overview
Catalog No.HS928014
Description
Anti-Human ACSM3 Polyclonal Antibody (HS928014) is a rabbit polyclonal antibody detecting ACSM3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ACSM3 (His302-Glu577).
Target Acyl-coenzyme A synthetase ACSM3, mitochondrial, 6.2.1.2, Acyl-CoA synthetase medium-chain family member 3, Butyrate--CoA ligase 3, Butyryl-coenzyme A synthetase 3, Middle-chain acyl-CoA synthetase 3, Propionate--CoA ligase, 6.2.1.17, Protein SA homolog, ACSM3, SAH
Purification Purified by antigen affinity column.
Accession Q53FZ2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Acyl-coenzyme A synthetase ACSM3, mitochondrial is a ~66 kDa protein. Catalyzes the activation of fatty acids by CoA to produce an acyl-CoA, the first step in fatty acid metabolism. Capable of activating medium-chain fatty acids with a preference for isobutyrate among fatty acids with 2-6 carbon atoms.

1. Iwai, N. et al. (2002) Circulation 105, 41-7. PMID: 11772874
Note For research use only.
Images
  • Anti-Human ACSM3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACSM3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate
    Lane 2: Mouse kidney lysate
    Lane 3: Rat liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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