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Anti-Human ACTL7A Polyclonal Antibody (HP966014)

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Anti-Human ACTL7A Polyclonal Antibody
Anti-Human ACTL7A Polyclonal Antibody
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Overview
Catalog No.HP966014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ACTL7A (Val72-Phe435).
Target Actin-like-7-alpha, ACTL7A, Actin-like protein 7A
Purification Purified by antigen affinity column.
Accession Q9Y615
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Actin-like protein 7A (ACTL7A) is a ~48 kDa protein. Essential for normal spermatogenesis and male fertility. Required for normal sperm head morphology, acroplaxome formation, acrosome attachment, and acrosome granule stability. May anchor and stabilize acrosomal adherence to the acroplaxome at least in part by facilitating the presence of F-actin in the subacrosomal space. May play an important role in formation and fusion of Golgi-derived vesicles during acrosome biogenesis.

1. Xin, A. et al. (2020) Science advances 6, eaaz4796. PMID: 32923619
Note For research use only.
Images
  • Anti-Human ACTL7A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACTL7A polyclonal antibody at 1μg/ml on various samples.
    Lane 1: U87 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Mouse liver lysate
    Lane 4: Mouse testis lysate
    Lane 5: Rat liver lysate
    Lane 6: Rat testis lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ACTL7A Polyclonal Antibody

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