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Anti-Human ACTN2 Polyclonal Antibody (HW330014)

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Anti-Human ACTN2 Polyclonal Antibody
Anti-Human ACTN2 Polyclonal Antibody
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Overview
Catalog No.HW330014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ACTN2 (Lys509-Leu894).
Target ACTN2, F-actin cross-linking protein, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2
Purification Purified by antigen affinity column.
Accession P35609
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-actinin-2 (ACTN2) is a ~103 kDa protein. F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Note For research use only.
Images
  • Anti-Human ACTN2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACTN2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: SH-SY5Y cell lysate
    Lane 2: C2C12 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: Mouse brain lysate
    Lane 6: Mouse lung lysate
    Lane 7: Mouse heart lysate
    Lane 8: Mouse muscle lysate
    Lane 9: Mouse kidney lysate
    Lane 10: Rat brain lysate
    Lane11: Rat lung lysate
    Lane12: Rat heart lysate
    Lane13: Rat muscle lysate
    Lane14: Rat kidney lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ACTN2 Polyclonal Antibody

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