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Anti-Human ADAM12 Recombinant Antibody (SAA1511), PE (HT428323)

Anti-Human ADAM12 Recombinant Antibody (SAA1511), PE
Anti-Human ADAM12 Recombinant Antibody (SAA1511), PE
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Overview
Catalog No.HT428323
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeVHH-mFc
Clone IDSAA1511
ConjugationPE
Clonality Monoclonal
Target ADAM 12, MLTN, Meltrin-alpha, Disintegrin and metalloproteinase domain-containing protein 12, ADAM12
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession O43184
RRID Anti-Human ADAM12 Antibody (SAA1511), PE (abinScience Cat# HT428323, RRID:AB_3729856)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) is a ~99 kDa protein. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human ADAM12 Recombinant Antibody (SAA1511), PE

    Flow cytometry

    Flow-cytometry using PE anti-human ADAM12 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAM12 monoclonal antibody (Catalog HT428323, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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