

| Catalog No. | HT428323 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | VHH-mFc |
| Clone ID | SAA1511 |
| Conjugation | PE |
| Clonality | Monoclonal |
| Target | ADAM 12, MLTN, Meltrin-alpha, Disintegrin and metalloproteinase domain-containing protein 12, ADAM12 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | O43184 |
| RRID | Anti-Human ADAM12 Antibody (SAA1511), PE (abinScience Cat# HT428323, RRID:AB_3729856) |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) is a ~99 kDa protein. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |

Flow-cytometry using PE anti-human ADAM12 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAM12 monoclonal antibody (Catalog HT428323, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.




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