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Anti-Human ADH4 Polyclonal Antibody (HY087014)

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Overview
Catalog No.HY087014
Description
Anti-Human ADH4 Polyclonal Antibody (HY087014) is a rabbit polyclonal antibody detecting ADH4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ADH4 (Met1-Phe380).
Target All-trans-retinol dehydrogenase [NAD(+)] ADH4, 1.1.1.105, Alcohol dehydrogenase 2, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH4, ADH2
Purification Purified by antigen affinity column.
Accession P08319
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

All-trans-retinol dehydrogenase [NAD(+)] ADH4 is a ~40 kDa protein. Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Also catalyzes the reduction of benzoquinones.

1. Hellgren, M. et al. (2007) Cellular and molecular life sciences : CMLS 64, 498-505. PMID: 17279314
2. Collins, XH. et al. (2005) The Journal of biological chemistry 280, 33157-64. PMID: 16081420
3. Svensson, S. et al. (1999) The Journal of biological chemistry 274, 29712-9. PMID: 10514444
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human ADH4 Polyclonal Antibody

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