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Anti-Human ADI1 Polyclonal Antibody (HP903014)

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Overview
Catalog No.HP903014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ADI1 (Met1-Ala179).
Target Sip-L, Acireductone dioxygenase (Fe(2+)-requiring), ADI1, ARD, 1, 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, MTCBP-1, Submergence-induced protein-like factor, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Fe-ARD, MTCBP1
Purification Purified by antigen affinity column.
Accession Q9BV57
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Acireductone dioxygenase (ADI1) is a ~21 kDa protein. Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.

1. Hirano, W. et al. (2005) Genes to cells : devoted to molecular & cellular mechanisms 10, 565-74. PMID: 15938715
2. Uekita, T. et al. (2004) The Journal of biological chemistry 279, 12734-43. PMID: 14718544
3. Yeh, CT. et al. (2001) Journal of virology 75, 11017-24. PMID: 11602742
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ADI1 Polyclonal Antibody

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