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Anti-Human AFF4 Polyclonal Antibody (HP615014)

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Overview
Catalog No.HP615014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AFF4 (Ser914-Lys1160).
Target Major CDK9 elongation factor-associated protein, MCEF, Protein AF-5q31, ALL1-fused gene from chromosome 5q31 protein, AF5Q31, AFF4, AF4/FMR2 family member 4
Purification Purified by antigen affinity column.
Accession Q9UHB7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

AF4/FMR2 family member 4 (AFF4) is a ~127 kDa protein. Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

Note For research use only.
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Formula
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Anti-Human AFF4 Polyclonal Antibody

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