Anti-Human AGXT Polyclonal Antibody (HB679014)

Name: Anti-Human AGXT Polyclonal Antibody
Brand: abinScience
SKU: HB679014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB679014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AGXT (Met1-Leu392).

Target

SPAT, Serine--pyruvate aminotransferase, AGXT, AGT1, Alanine--glyoxylate aminotransferase, AGT, SPT

Purification

Purified by antigen affinity column.

Accession

P21549

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Alanine--glyoxylate aminotransferase (AGXT) is a ~43 kDa protein. Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification. Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism.

1. Lumb, MJ. et al. (2000) The Journal of biological chemistry 275, 36415-22. PMID: 10960483

2. Santana, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 7277-82. PMID: 12777626

3. Fargue, S. et al. (2013) The Journal of biological chemistry 288, 2475-84. PMID: 23229545

4. Oppici, E. et al. (2013) Biochimica et biophysica acta 1832, 2277-88. PMID: 24055001

5. Montioli, R. et al. (2015) Biochimica et biophysica acta 1854, 1280-9. PMID: 26149463

6. Xue, HH. et al. (1999) The Journal of biological chemistry 274, 16028-33. PMID: 10347152

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AGXT polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate

References

Datasheet

Anti-Human AGXT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human AGXT Polyclonal Antibody (HB679014)

Overview

Images

References

Datasheet

SDS

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