Anti-Human AIRE Polyclonal Antibody (HT425014)

Name: Anti-Human AIRE Polyclonal Antibody
Brand: abinScience
SKU: HT425014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT425014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AIRE (Ala2-Asp105).

Target

AIRE, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator, APECED, APECED protein

Purification

Purified by antigen affinity column.

Accession

O43918

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Autoimmune regulator (AIRE) is a ~57 kDa protein. Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression.

1. Oftedal, BE. et al. (2015) Immunity 42, 1185-96. PMID: 26084028

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human AIRE polyclonal antibody at 1ug/mL on recombinant Human AIRE (Catalog No. HT425012).

References

Datasheet

Anti-Human AIRE Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human AIRE Polyclonal Antibody (HT425014)

Overview

Images

References

Datasheet

SDS

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