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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014)

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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
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Overview
Catalog No.HF865014
Description
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014) is a rabbit polyclonal antibody detecting AK1/Adenylate kinase isoenzyme 1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AK1/Adenylate kinase isoenzyme 1 (Met1-Lys194).
Target AK1/Adenylate kinase isoenzyme 1
Purification Purified by antigen affinity column.
Accession P00568
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesAdenylate kinase isoenzyme 1, AK 1, 2.7.4.3, 2.7.4.6, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase, AK1
Background

Adenylate kinase isoenzyme 1 (AK1) is a ~21 kDa protein. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP.

1. Panayiotou, C. et al. (2011) The Biochemical journal 433, 527-34. PMID: 21080915
2. Amiri, M. et al. (2013) The international journal of biochemistry & cell biology 45, 925-31. PMID: 23416111
3. Matsuura, S. et al. (1989) The Journal of biological chemistry 264, 10148-55. PMID: 2542324
Note For research use only.
Images
  • Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AK1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse skeletal muscle lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat skeletal muscle lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody

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