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Anti-Human AKAP3 Polyclonal Antibody (HF991014)

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Overview
Catalog No.HF991014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human AKAP3 (Ala726-Leu853).
Target Fibrousheathin I, AKAP110, CT82, Fibrous sheath protein of 95 kDa, FSP95, Fibrousheathin-1, A-kinase anchor protein 110 kDa, AKAP3, Protein kinase A-anchoring protein 3, Cancer/testis antigen 82, AKAP 110, AKAP-3, Sperm oocyte-binding protein, SOB1, A-kinase anchor protein 3, PRKA3
Purification Purified by antigen affinity column.
Accession O75969
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

A-kinase anchor protein 3 (AKAP3) is a ~94 kDa protein. Structural component of sperm fibrous sheath. Required for the formation of the subcellular structure of the sperm flagellum, sperm motility and male fertility.

1. Liu, C. et al. (2023) Journal of medical genetics 60, 137-143. PMID: 35228300
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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