Anti-Human AKR1C2 Polyclonal Antibody (HA716014)

Name: Anti-Human AKR1C2 Polyclonal Antibody
Brand: abinScience
SKU: HA716014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA716014

Description

Anti-Human AKR1C2 Polyclonal Antibody (HA716014) is a rabbit polyclonal antibody detecting Aldo-keto reductase family 1 member C2; 1.-.-.-; 1.1.1.112; 1.1.1.209; 1.1.1.53; 1.1.1.62; 1.3.1.20; 3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Type III 3-alpha-hydroxysteroid dehydrogenase; 1.1.1.357; AKR1C2; DDH2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human AKR1C2 (Met1-Tyr323).

Target

Aldo-keto reductase family 1 member C2; 1.-.-.-; 1.1.1.112; 1.1.1.209; 1.1.1.53; 1.1.1.62; 1.3.1.20; 3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Type III 3-alpha-hydroxysteroid dehydrogenase; 1.1.1.357; AKR1C2; DDH2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P52895, Q04828, P42330

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Aldo-keto reductase family 1 member C2 (AKR1C2) is a ~36 kDa protein. Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 3alpha-hydroxysteroids, but also 3beta-, 17beta- and 20alpha-hydroxysteroids. Required for male sex determination as a component of the 'backdoor' androgen biosynthesis pathway that generates 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes. Acts together with AKR1C4 to convert 5alpha-dihydroprogesterone (5alpha-DHP) to 3alpha-hydroxy-5alpha-pregnan-20-one (3alpha,5alpha-THP/allopregnanolone), leading to 5alpha-DHT secretion necessary for embryonic gonad differentiation into testis. In androgen catabolism, may predominantly act as a phase I enzyme by introducing a hydroxyl group prior to conjugation.

1. Dufort, I. et al. (1996) Biochemical and biophysical research communications 228, 474-9. PMID: 8920937

2. Shiraishi, H. et al. (1998) The Biochemical journal 334 ( Pt 2), 399-405. PMID: 9716498

3. Penning, TM. et al. (2000) The Biochemical journal 351, 67-77. PMID: 10998348

4. Trauger, JW. et al. (2002) Biochemistry 41, 13451-9. PMID: 12416991

5. Usami, N. et al. (2002) Biological & pharmaceutical bulletin 25, 441-5. PMID: 11995921

6. Higaki, Y. et al. (2003) Chemico-biological interactions 143-144, 503-13. PMID: 12604236

7. Steckelbroeck, S. et al. (2004) The Journal of biological chemistry 279, 10784-95. PMID: 14672942

8. Jin, Y. et al. (2009) The Journal of biological chemistry 284, 10013-22. PMID: 19218247

9. Flück, CE. et al. (2011) American journal of human genetics 89, 201-18. PMID: 21802064

10. Nahoum, V. et al. (2001) The Journal of biological chemistry 276, 42091-8. PMID: 11514561

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-AKR1C2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-Human AKR1C2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human AKR1C2 Polyclonal Antibody (HA716014)

Overview

Images

References

Datasheet

SDS

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