Anti-Human ALPK1 Polyclonal Antibody (HD668014)

Name: Anti-Human ALPK1 Polyclonal Antibody
Brand: abinScience
SKU: HD668014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HD668014

Description

Anti-Human ALPK1 Polyclonal Antibody (HD668014) is a rabbit polyclonal antibody detecting ALPK1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALPK1 (Ser960-Thr1244).

Target

ALPK1, Alpha-protein kinase 1, Chromosome 4 kinase, EC:2.7.11.1, KIAA1527, LAK, Lymphocyte alpha-protein kinase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q96QP1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Alpha-protein kinase 1 (ALPK1) is a ~138 kDa protein. Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity. Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria. ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling. May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A. May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A.

1. Milivojevic, M. et al. (2017) PLoS pathogens 13, e1006224. PMID: 28222186

2. Zimmermann, S. et al. (2017) Cell reports 20, 2384-2395. PMID: 28877472

3. Zhou, P. et al. (2018) Nature 561, 122-126. PMID: 30111836

4. Kozycki, CT. et al. (2022) Annals of the rheumatic diseases 81, 1453-1464. PMID: 35868845

5. Snelling, T. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2313148120. PMID: 38060563

6. Lee, CP. et al. (2016) Scientific reports 6, 25740. PMID: 27169898

7. Heine, M. et al. (2005) The Journal of biological chemistry 280, 25637-43. PMID: 15883161

8. Williams, LB. et al. (2019) Genetics in medicine : official journal of the American College of Medical Genetics 21, 2103-2115. PMID: 30967659

Note

For research use only

Images

References

Datasheet

Anti-Human ALPK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ALPK1 Polyclonal Antibody (HD668014)

Overview

Images

References

Datasheet

SDS

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