Anti-Human APH1A Polyclonal Antibody (HP487024)

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP487024

Description

Anti-Human APH1A Polyclonal Antibody (HP487024) is a rabbit polyclonal antibody detecting Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A; PSF; CGI-78; UNQ579/PRO1141 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human APH1A (Trp53-Gln68&Tyr90-Ala118&Asp140-Leu158&Ser235-Asp265).

Target

Gamma-secretase subunit APH-1A; APH-1a; Aph-1alpha; Presenilin-stabilization factor; APH1A; PSF; CGI-78; UNQ579/PRO1141

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q96BI3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Gamma-secretase subunit APH-1A (APH1A) is a ~28 kDa protein. Non-catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Required for normal gamma-secretase assembly. The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable).

1. Lee, SF. et al. (2002) The Journal of biological chemistry 277, 45013-9. PMID: 12297508

2. Luo, WJ. et al. (2003) The Journal of biological chemistry 278, 7850-4. PMID: 12522139

3. Edbauer, D. et al. (2003) Nature cell biology 5, 486-8. PMID: 12679784

4. Marlow, L. et al. (2003) Biochemical and biophysical research communications 305, 502-9. PMID: 12763021

5. Lu, P. et al. (2014) Nature 512, 166-170. PMID: 25043039

6. Bai, XC. et al. (2015) Nature 525, 212-217. PMID: 26280335

7. Yang, G. et al. (2019) Nature 565, 192-197. PMID: 30598546

8. Zhou, R. et al. (2019) Science (New York, N.Y.) 363. PMID: 30630874

9. Gu, Y. et al. (2003) The Journal of biological chemistry 278, 7374-80. PMID: 12471034

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-Human APH1A polyclonal antibody at 1ug/mL on recombinant Human APH1A (Catalog No. HP487022).

References

Datasheet