Anti-Human APOBEC3H Polyclonal Antibody (HB477014)

Name: Anti-Human APOBEC3H Polyclonal Antibody
Brand: abinScience
SKU: HB477014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB477014

Description

Anti-Human APOBEC3H Polyclonal Antibody (HB477014) is a rabbit polyclonal antibody detecting DNA dC->dU-editing enzyme APOBEC-3H; 3.5.4.38; APOBEC-related protein 10; ARP-10; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H; A3H; APOBEC3H in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human APOBEC3H (Met1-Val200).

Target

DNA dC->dU-editing enzyme APOBEC-3H; 3.5.4.38; APOBEC-related protein 10; ARP-10; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H; A3H; APOBEC3H

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6NTF7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA dC->dU-editing enzyme APOBEC-3H (APOBEC3H) is a ~23 kDa protein. DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. The A3H-var/haplotype 2 exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA.

1. OhAinle, M. et al. (2006) Journal of virology 80, 3853-62. PMID: 16571802

2. Dang, Y. et al. (2006) Journal of virology 80, 10522-33. PMID: 16920826

3. OhAinle, M. et al. (2008) Cell host & microbe 4, 249-59. PMID: 18779051

4. Tan, L. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 279-87. PMID: 18827027

5. Stenglein, MD. et al. (2010) Nature structural & molecular biology 17, 222-9. PMID: 20062055

6. Phalora, PK. et al. (2012) Journal of virology 86, 11712-24. PMID: 22915799

7. Shaban, NM. et al. (2018) Molecular cell 69, 75-86.e9. PMID: 29290613

8. Dang, Y. et al. (2008) The Journal of biological chemistry 283, 11606-14. PMID: 18299330

9. Hultquist, JF. et al. (2011) Journal of virology 85, 11220-34. PMID: 21835787

10. Chaipan, C. et al. (2013) Journal of virology 87, 444-53. PMID: 23097438

Note

For research use only

Images

References

Datasheet

Anti-Human APOBEC3H Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human APOBEC3H Polyclonal Antibody (HB477014)

Overview

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Datasheet

SDS

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