Human APOBEC3H Recombinant Protein (N-His) (HB477012)

Name: Human APOBEC3H Recombinant Protein (N-His)
Brand: abinScience
SKU: HB477012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB477012
Description	Recombinant Human APOBEC3H Protein, N-His (HB477012) expressed in E. coli, spanning Met1-Val200. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6NTF7
Protein length	Met1-Val200
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.70 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA dC->dU-editing enzyme APOBEC-3H; 3.5.4.38; APOBEC-related protein 10; ARP-10; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3H; A3H; APOBEC3H
Background	DNA dC->dU-editing enzyme APOBEC-3H is a ~23 kDa protein. DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. The A3H-var/haplotype 2 exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. 1. OhAinle, M. et al. (2006) Journal of virology 80, 3853-62. PMID: 16571802 2. Dang, Y. et al. (2006) Journal of virology 80, 10522-33. PMID: 16920826 3. OhAinle, M. et al. (2008) Cell host & microbe 4, 249-59. PMID: 18779051 4. Tan, L. et al. (2009) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 23, 279-87. PMID: 18827027 5. Stenglein, MD. et al. (2010) Nature structural & molecular biology 17, 222-9. PMID: 20062055 6. Phalora, PK. et al. (2012) Journal of virology 86, 11712-24. PMID: 22915799 7. Shaban, NM. et al. (2018) Molecular cell 69, 75-86.e9. PMID: 29290613 8. Dang, Y. et al. (2008) The Journal of biological chemistry 283, 11606-14. PMID: 18299330 9. Hultquist, JF. et al. (2011) Journal of virology 85, 11220-34. PMID: 21835787 10. Chaipan, C. et al. (2013) Journal of virology 87, 444-53. PMID: 23097438
Note	For research use only

Images

SDS-PAGE

SDS-PAGE for Recombinant Human APOBEC3H Protein, N-His

References

Datasheet

Human APOBEC3H Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human APOBEC3H Recombinant Protein (N-His) (HB477012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us