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Anti-Human AQP8 Polyclonal Antibody (HC039014)

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Overview
Catalog No.HC039014
Description
Anti-Human AQP8 Polyclonal Antibody (HC039014) is a rabbit polyclonal antibody detecting Aquaporin-8; AQP-8; AQP8 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human AQP8 (Glu58-Gly84&Lys129-Gly156&Ser205-Trp228).
Target Aquaporin-8; AQP-8; AQP8
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O94778
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aquaporin-8 (AQP8) is a ~27 kDa protein. Channel that allows the facilitated permeation of water and uncharged molecules, such as hydrogen peroxide and the neutral form of ammonia (NH3), through cellular membranes such as plasma membrane, inner mitochondrial membrane and endoplasmic reticulum membrane of several tissues. The transport of the ammonia neutral form induces a parallel transport of proton, at alkaline pH when the concentration of ammonia is high. However, it is unclear whether the transport of proton takes place via the aquaporin or via an endogenous pathway. Also, may transport ammonia analogs such as formamide and methylamine, a transport favourited at basic pH due to the increase of unprotonated (neutral) form, which is expected to favor diffusion. Does not transport urea or glycerol.

1. Liu, K. et al. (2006) Biology of the cell 98, 153-61. PMID: 15948717
2. Larocca, MC. et al. (2009) American journal of physiology. Gastrointestinal and liver physiology 296, G93-100. PMID: 18948439
3. Bertolotti, M. et al. (2013) Antioxidants & redox signaling 19, 1447-51. PMID: 23541115
4. Medraño-Fernandez, I. et al. (2016) Antioxidants & redox signaling 24, 1031-44. PMID: 26972385
5. Bestetti, S. et al. (2018) Science advances 4, eaar5770. PMID: 29732408
6. Danielli, M. et al. (2019) Free radical biology & medicine 131, 370-375. PMID: 30579780
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human AQP8 Polyclonal Antibody

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