Anti-Human ARF4 Polyclonal Antibody (HB009014)

Name: Anti-Human ARF4 Polyclonal Antibody
Brand: abinScience
SKU: HB009014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB009014

Description

Anti-Human ARF4 Polyclonal Antibody (HB009014) is a rabbit polyclonal antibody detecting ARF4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARF4 (Met1-Arg180).

Target

ADP-ribosylation factor 4, ARF4, ARF2

Purification

Purified by antigen affinity column.

Accession

P18085

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ADP-ribosylation factor 4 (ARF4) is a ~20 kDa protein. GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. Part of the ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation.

1. Wang, J. et al. (2015) Journal of cell science 128, 1375-85. PMID: 25673879

Note

For research use only.

Images

References

Datasheet

Anti-Human ARF4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ARF4 Polyclonal Antibody (HB009014)

Overview

Images

References

Datasheet

SDS

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