Anti-Human ARFGAP1 Polyclonal Antibody (HP401014)

Name: Anti-Human ARFGAP1 Polyclonal Antibody
Brand: abinScience
SKU: HP401014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP401014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARFGAP1 (Met1-Gly121).

Target

ARF1 GAP, ARF1GAP, ADP-ribosylation factor GTPase-activating protein 1, ARFGAP1, ADP-ribosylation factor 1 GTPase-activating protein, ARF GAP 1, ARF1-directed GTPase-activating protein

Purification

Purified by antigen affinity column.

Accession

Q8N6T3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ADP-ribosylation factor GTPase-activating protein 1 (ARFGAP1) is a ~44 kDa protein. GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human ARFGAP1 polyclonal antibody at 1ug/mL on recombinant Human ARFGAP1 (Catalog No. HP401012).

References

Datasheet

Anti-Human ARFGAP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ARFGAP1 Polyclonal Antibody (HP401014)

Overview

Images

References

Datasheet

SDS

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