Anti-Human ARHGDIA Polyclonal Antibody (HW313014)

Name: Anti-Human ARHGDIA Polyclonal Antibody
Brand: abinScience
SKU: HW313014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW313014

Description

Anti-Human ARHGDIA Polyclonal Antibody (HW313014) is a rabbit polyclonal antibody detecting ARHGDIA in ELISA, IHC, WB. Suitable for Bovine, Human, Macaca fascicularis, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ARHGDIA (Asn69-Asp204).

Target

Rho GDP-dissociation inhibitor 1, GDIA1, Rho GDI 1, Rho-GDI alpha, ARHGDIA

Purification

Purified by antigen affinity column.

Accession

P52565

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Rho GDP-dissociation inhibitor 1 (ARHGDIA) is a ~23 kDa protein. Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ARHGDIA polyclonal antibody at 1μg/ml on various samples.
Lane 1: HL-60 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Mouse lung lysate
Lane 4: Rat lung lysate

References

Datasheet

Anti-Human ARHGDIA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ARHGDIA Polyclonal Antibody (HW313014)

Overview

Images

References

Datasheet

SDS

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