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Anti-Human ARHGEF12 Polyclonal Antibody (HA489014)

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Overview
Catalog No.HA489014
Description
Anti-Human ARHGEF12 Polyclonal Antibody (HA489014) is a rabbit polyclonal antibody detecting ARHGEF12 in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ARHGEF12 (Asn366-Gly556).
Target ARHGEF12
Purification Purified by antigen affinity column.
Accession Q9NZN5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesRho guanine nucleotide exchange factor 12, Leukemia-associated RhoGEF, ARHGEF12
Background

Rho guanine nucleotide exchange factor 12 (ARHGEF12) is a ~173 kDa protein. Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Promotes endothelial cell and actin stress fiber reorientation in response to mechanotransduction.

1. Fukuhara, S. et al. (2000) FEBS letters 485, 183-8. PMID: 11094164
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ARHGEF12 Polyclonal Antibody

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