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Anti-Human ARHGEF25 Polyclonal Antibody (HP257014)

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Overview
Catalog No.HP257014
Description
Anti-Human ARHGEF25 Polyclonal Antibody (HP257014) is a rabbit polyclonal antibody detecting ARHGEF25 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ARHGEF25 (Ser149-Ser490).
Target Rho guanine nucleotide exchange factor 25, p63RhoGEF, GEFT, Guanine nucleotide exchange factor GEFT, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT, ARHGEF25
Purification Purified by antigen affinity column.
Accession Q86VW2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Rho guanine nucleotide exchange factor 25 (ARHGEF25) is a ~63 kDa protein. May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ARHGEF25 Polyclonal Antibody

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