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Anti-Human ARID5B Polyclonal Antibody (HD669014)

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Overview
Catalog No.HD669014
Description
Anti-Human ARID5B Polyclonal Antibody (HD669014) is a rabbit polyclonal antibody detecting ARID5B. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ARID5B (Met1-Asn247).
Target ARID domain-containing protein 5B, ARID5B, AT-rich interactive domain-containing protein 5B, DESRT, MRF-2, MRF1-like protein, MRF2, Modulator recognition factor 2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q14865
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

AT-rich interactive domain-containing protein 5B (ARID5B) is a ~132 kDa protein. Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-336, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation.

Note For research use only
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Formula
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Anti-Human ARID5B Polyclonal Antibody

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