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Anti-Human ARPC3 Polyclonal Antibody (HT287014)

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Overview
Catalog No.HT287014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ARPC3 (Asp40-Gln178).
Target Arp2/3 complex 21 kDa subunit, p21-ARC, ARPC3, Actin-related protein 2/3 complex subunit 3, ARC21
Purification Purified by antigen affinity column.
Accession O15145
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Actin-related protein 2/3 complex subunit 3 (ARPC3) is a ~20 kDa protein. Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

1. Welch, MD. et al. (1997) The Journal of cell biology 138, 375-84. PMID: 9230079
2. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947
Note For research use only.
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Formula
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Anti-Human ARPC3 Polyclonal Antibody

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