Anti-Human ARRB2 Polyclonal Antibody (HB908014)

Name: Anti-Human ARRB2 Polyclonal Antibody
Brand: abinScience
SKU: HB908014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB908014

Description

Anti-Human ARRB2 Polyclonal Antibody (HB908014) is a rabbit polyclonal antibody detecting ARRB2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ARRB2 (Met1-Pro368).

Target

Beta-arrestin-2, Arrestin beta-2, Non-visual arrestin-3, ARRB2, ARB2, ARR2

Purification

Purified by antigen affinity column.

Accession

P32121

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Beta-arrestin-2 (ARRB2) is a ~46 kDa protein. Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation. The beta-arrestins target many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins) and recruiting the GPRCs to the adapter protein 2 complex 2 (AP-2) in clathrin-coated pits (CCPs). However, the extent of beta-arrestin involvement appears to vary significantly depending on the receptor, agonist and cell type. Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins.

1. Maharana, J. et al. (2023) Molecular cell 83, 2091-2107.e7. PMID: 37209686

2. Maharana, J. et al. (2024) Science (New York, N.Y.) 383, 101-108. PMID: 38175886

3. Jean-Charles, PY. et al. (2016) The Journal of biological chemistry 291, 7450-64. PMID: 26839314

4. Watson, SJ. et al. (2012) Molecular pharmacology 81, 631-42. PMID: 22282525

5. Yan, Y. et al. (2013) Immunity 38, 1154-63. PMID: 23809162

Note

For research use only.

Images

References

Datasheet

Anti-Human ARRB2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human ARRB2 Polyclonal Antibody (HB908014)

Overview

Images

References

Datasheet

SDS

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