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Anti-Human ASH2L Polyclonal Antibody (HP309014)

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Overview
Catalog No.HP309014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ASH2L (Met430-Pro628).
Target ASH2L, ASH2-like protein, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2L1
Purification Purified by antigen affinity column.
Accession Q9UBL3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Set1/Ash2 histone methyltransferase complex subunit ASH2 (ASH2L) is a ~68 kDa protein. Transcriptional regulator. Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters. Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May play a role in hematopoiesis.

1. Wysocka, J. et al. (2003) Genes & development 17, 896-911. PMID: 12670868
2. Garapaty, S. et al. (2009) The Journal of biological chemistry 284, 7542-52. PMID: 19131338
3. Patel, A. et al. (2009) The Journal of biological chemistry 284, 24242-56. PMID: 19556245
4. Avdic, V. et al. (2011) Structure (London, England : 1993) 19, 101-8. PMID: 21220120
5. Zhang, P. et al. (2012) Nucleic acids research 40, 4237-46. PMID: 22266653
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ASH2L Polyclonal Antibody

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