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Anti-Human ATG10 Polyclonal Antibody (HB377014)

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Overview
Catalog No.HB377014
Description
Anti-Human ATG10 Polyclonal Antibody (HB377014) is a rabbit polyclonal antibody detecting Ubiquitin-like-conjugating enzyme ATG10; 2.3.2.-; Autophagy-related protein 10; APG10-like; ATG10; APG10L; PP12616 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human ATG10 (Met1-Pro220).
Target Ubiquitin-like-conjugating enzyme ATG10; 2.3.2.-; Autophagy-related protein 10; APG10-like; ATG10; APG10L; PP12616
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9H0Y0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-like-conjugating enzyme ATG10 is a ~25 kDa protein. E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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