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Anti-Human ATP10A Polyclonal Antibody (HT395014)

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Overview
Catalog No.HT395014
Description
Anti-Human ATP10A Polyclonal Antibody (HT395014) is a rabbit polyclonal antibody detecting ATP10A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ATP10A (Glu688-Arg866).
Target Phospholipid-transporting ATPase VA, 7.6.2.1, ATPase class V type 10A, Aminophospholipid translocase VA, P4-ATPase flippase complex alpha subunit ATP10A, ATP10A, ATP10C, ATPVA, ATPVC, KIAA0566
Purification Purified by antigen affinity column.
Accession O60312
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phospholipid-transporting ATPase VA (ATP10A) is a ~167 kDa protein. Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane. Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking. Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix. Has low flippase activity toward glucosylceramide (GlcCer).

1. Naito, T. et al. (2015) The Journal of biological chemistry 290, 15004-17. PMID: 25947375
2. Takada, N. et al. (2018) The EMBO journal 37. PMID: 29599178
3. Roland, BP. et al. (2019) The Journal of biological chemistry 294, 1794-1806. PMID: 30530492
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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