Anti-Human ATP5F1A Polyclonal Antibody (HD604014)

Name: Anti-Human ATP5F1A Polyclonal Antibody
Brand: abinScience
SKU: HD604014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD604014

Description

Anti-Human ATP5F1A Polyclonal Antibody (HD604014) is a rabbit polyclonal antibody detecting ATP5F1A. Suitable for Rat, Human, and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP5F1A (Gln44-Ala553).

Target

ATP synthase F(1) complex subunit alpha, mitochondrial, ATP synthase F1 subunit alpha, ATP5A, ATP5A1, ATP5AL2, ATP5F1A, ATPM

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P25705

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

ATP synthase F(1) complex subunit alpha, mitochondrial (ATP5F1A) is a ~59 kDa protein. Subunit alpha, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (Probable). ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro.

1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256

2. Qi, B. et al. (2018) Cell 175, 571-582.e11. PMID: 30146159

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-ATP5F1A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

References

Datasheet

Anti-Human ATP5F1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP5F1A Polyclonal Antibody (HD604014)

Overview

Images

References

Datasheet

SDS

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