Anti-Human ATP6V1B1 Polyclonal Antibody (HB149014)

Name: Anti-Human ATP6V1B1 Polyclonal Antibody
Brand: abinScience
SKU: HB149014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB149014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATP6V1B1 (Asp111-Leu513).

Target

ATP6V1B1, Endomembrane proton pump 58 kDa subunit, V-ATPase subunit B 1, Vacuolar proton pump subunit B 1, VPP3, V-type proton ATPase subunit B, kidney isoform, VATB, ATP6B1

Purification

Purified by antigen affinity column.

Accession

P15313

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

V-type proton ATPase subunit B, kidney isoform (ATP6V1B1) is a ~56 kDa protein. Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium.

1. Yang, Q. et al. (2006) Journal of the American Society of Nephrology : JASN 17, 1858-66. PMID: 16769747

2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ATP6V1B1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse kidney lysate
Lane 3: Rat brain lysate

References

Datasheet

Anti-Human ATP6V1B1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP6V1B1 Polyclonal Antibody (HB149014)

Overview

Images

References

Datasheet

SDS

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