Anti-Human ATP6V1D Polyclonal Antibody (HP356014)

Name: Anti-Human ATP6V1D Polyclonal Antibody
Brand: abinScience
SKU: HP356014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP356014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP6V1D (Met1-Glu247).

Target

ATP6M, V-ATPase subunit D, Vacuolar proton pump subunit D, VATD, ATP6V1D, V-type proton ATPase subunit D, V-ATPase 28 kDa accessory protein

Purification

Purified by antigen affinity column.

Accession

Q9Y5K8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

V-type proton ATPase subunit D (ATP6V1D) is a ~28 kDa protein. Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium.

1. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

2. Chen, Y. et al. (2012) Cell research 22, 333-45. PMID: 21844891

Note

For research use only.

Images

References

Datasheet

Anti-Human ATP6V1D Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP6V1D Polyclonal Antibody (HP356014)

Overview

Images

References

Datasheet

SDS

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