Anti-Human ATP6V1G1 Polyclonal Antibody (HF937014)

Name: Anti-Human ATP6V1G1 Polyclonal Antibody
Brand: abinScience
SKU: HF937014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HF937014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATP6V1G1 (Met1-Gly118).

Target

ATP6V1G1, Vacuolar proton pump subunit M16, ATP6G, V-type proton ATPase subunit G 1, Vacuolar proton pump subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, ATP6G1, ATP6J

Purification

Purified by antigen affinity column.

Accession

O75348

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

V-type proton ATPase subunit G 1 (ATP6V1G1) is a ~13 kDa protein. Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation.

1. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091

2. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002

3. Miles, AL. et al. (2017) eLife 6. PMID: 28296633

Note

For research use only.

Images

References

Datasheet

Anti-Human ATP6V1G1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATP6V1G1 Polyclonal Antibody (HF937014)

Overview

Images

References

Datasheet

SDS

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