Anti-Human ATXN3 Polyclonal Antibody (HX010014)

Name: Anti-Human ATXN3 Polyclonal Antibody
Brand: abinScience
SKU: HX010014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX010014

Description

Anti-Human ATXN3 Polyclonal Antibody (HX010014) is a rabbit polyclonal antibody detecting ATXN3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ATXN3 (Met1-Arg182).

Target

ATX3, MJD1, MJD, SCA3, Ataxin-3, Spinocerebellar ataxia type 3 protein, ATXN3, Machado-Joseph disease protein 1

Purification

Purified by antigen affinity column.

Accession

P54252

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ataxin-3 (ATXN3) is a ~41 kDa protein. Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. Acts as a negative regulator of mTORC1 signaling in response to amino acid deprivation by mediating deubiquitination of RHEB, thereby promoting RHEB inactivation by the TSC-TBC complex.

1. Li, F. et al. (2002) The Journal of biological chemistry 277, 45004-12. PMID: 12297501

2. Mao, Y. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 12700-5. PMID: 16118278

3. Tzvetkov, N. et al. (2007) Biological chemistry 388, 973-8. PMID: 17696782

4. Seki, T. et al. (2013) The Journal of biological chemistry 288, 17145-55. PMID: 23625928

5. Ashkenazi, A. et al. (2017) Nature 545, 108-111. PMID: 28445460

6. Yao, Y. et al. (2020) Molecular cell 80, 437-451.e6. PMID: 33157014

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ATXN3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate

References

Datasheet

Anti-Human ATXN3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ATXN3 Polyclonal Antibody (HX010014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us