Anti-Human BHLHE40 Polyclonal Antibody (HT136014)

Name: Anti-Human BHLHE40 Polyclonal Antibody
Brand: abinScience
SKU: HT136014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HT136014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human BHLHE40 (Ser46-Gly186).

Target

DEC1, bHLHe40, Differentially expressed in chondrocytes protein 1, Class E basic helix-loop-helix protein 40, Class B basic helix-loop-helix protein 2, STRA13, SHARP2, SHARP-2, bHLHb2, Enhancer-of-split and hairy-related protein 2, BHLHB2, BHLHE40, Stimulated by retinoic acid gene 13 protein

Purification

Purified by antigen affinity column.

Accession

O14503

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Class E basic helix-loop-helix protein 40 (BHLHE40) is a ~45 kDa protein. Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2.

1. Honma, S. et al. (2002) Nature 419, 841-4. PMID: 12397359

2. Nakashima, A. et al. (2008) Molecular and cellular biology 28, 4080-92. PMID: 18411297

3. Kawamoto, T. et al. (2004) Biochemical and biophysical research communications 313, 117-24. PMID: 14672706

4. Li, Y. et al. (2004) The Biochemical journal 382, 895-904. PMID: 15193144

5. Sato, F. et al. (2004) European journal of biochemistry 271, 4409-19. PMID: 15560782

6. Cho, Y. et al. (2009) Molecular pharmacology 76, 1360-9. PMID: 19786558

7. Marczak, MM. et al. (2017) Archives of biochemistry and biophysics 631, 11-18. PMID: 28797635

8. Nakashima, A. et al. (2018) Hypertension (Dallas, Tex. : 1979) 72, 746-754. PMID: 30012868

Note

For research use only.

Images

References

Datasheet

Anti-Human BHLHE40 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human BHLHE40 Polyclonal Antibody (HT136014)

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