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Anti-Human BRCA2 Polyclonal Antibody (HW695014)

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Overview
Catalog No.HW695014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human BRCA2 (Lys2308-His2537).
Target BRCA2, FANCD1, FACD, Fanconi anemia group D1 protein, Breast cancer type 2 susceptibility protein
Purification Purified by antigen affinity column.
Accession P51587
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Breast cancer type 2 susceptibility protein (BRCA2) is a ~384 kDa protein. Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation.

1. Bhatia, V. et al. (2014) Nature 511, 362-5. PMID: 24896180
Note For research use only.
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Formula
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Anti-Human BRCA2 Polyclonal Antibody

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