Anti-Human BUB1B Recombinant Antibody (SAA1518) (HT375013)

Name: Anti-Human BUB1B Recombinant Antibody (SAA1518)
Brand: abinScience
SKU: HT375013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT375013

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1518

Clonality

Monoclonal

Target

SSK1, MAD3/BUB1-related protein kinase, BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUBR1, MAD3L, Protein SSK1, hBUBR1, Mitotic checkpoint kinase MAD3L

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O60566

RRID

Anti-Human BUB1B Antibody (SAA1518) (abinScience Cat# HT375013, RRID:AB_3727279)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B) is a ~119 kDa protein. Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human BUB1B Antibody (SAA1518).

References

Datasheet

Anti-Human BUB1B Recombinant Antibody (SAA1518).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human BUB1B Recombinant Antibody (SAA1518) (HT375013)

Overview

Images

References

Datasheet

SDS

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