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Anti-Human CAMLG Polyclonal Antibody (HW596014)

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Anti-Human CAMLG Polyclonal Antibody
Anti-Human CAMLG Polyclonal Antibody
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Overview
Catalog No.HW596014
Description
Anti-Human CAMLG Polyclonal Antibody (HW596014) is a rabbit polyclonal antibody detecting CAMLG in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CAMLG (Gly19-Glu184).
Target CAMLG
Purification Purified by antigen affinity column.
Accession P49069
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesGuided entry of tail-anchored proteins factor CAMLG, Calcium signal-modulating cyclophilin ligand, CAMLG, CAML, GET2
Background

Guided entry of tail-anchored proteins factor CAMLG is a ~32 kDa protein. Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET1/WRB, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. Required for the stability of GET1. Stimulates calcium signaling in T cells through its involvement in elevation of intracellular calcium. Essential for the survival of peripheral follicular B cells.

1. Yamamoto, Y. et al. (2012) Molecular cell 48, 387-97. PMID: 23041287
2. Vilardi, F. et al. (2014) PloS one 9, e85033. PMID: 24392163
3. Colombo, SF. et al. (2016) The Journal of biological chemistry 291, 15292-306. PMID: 27226539
4. Inglis, AJ. et al. (2020) Cell reports 30, 3691-3698.e5. PMID: 32187542
5. Bram, RJ. et al. (1994) Nature 371, 355-8. PMID: 7522304
Note For research use only.
Images
  • Anti-Human CAMLG Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CAMLG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: 293T cell lysate
    Lane 3: Mouse testis lysate
    Lane 4: Mouse brain lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human CAMLG Polyclonal Antibody

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