Anti-Human CARM1 Polyclonal Antibody (HP765014)

Name: Anti-Human CARM1 Polyclonal Antibody
Brand: abinScience
SKU: HP765014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP765014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CARM1 (Thr28-Asn471).

Target

Histone-arginine methyltransferase CARM1, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4, Protein arginine N-methyltransferase 4

Purification

Purified by antigen affinity column.

Accession

Q86X55

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone-arginine methyltransferase CARM1 is a ~65 kDa protein. Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activation of transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B.

1. Li, H. et al. (2002) The Journal of biological chemistry 277, 44623-30. PMID: 12237300

2. Miao, F. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 1562-73. PMID: 16497732

3. Lakowski, TM. et al. (2009) The Biochemical journal 421, 253-61. PMID: 19405910

4. Lee, YH. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 3611-6. PMID: 15731352

5. Genois, MM. et al. (2021) Molecular cell 81, 784-800.e8. PMID: 33412112

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CARM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse liver lysate
Lane 2: Rat liver lysate

References

Datasheet

Anti-Human CARM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human CARM1 Polyclonal Antibody (HP765014)

Overview

Images

References

Datasheet

SDS

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