Anti-Human CBX4 Recombinant Antibody (P004) (HT334013)

Name: Anti-Human CBX4 Recombinant Antibody (P004)
Brand: abinScience
SKU: HT334013
Price: 191 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT334013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG2a

Clone ID

P004

Clonality

Monoclonal

Target

CBX4, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O00257

RRID

Anti-Human CBX4 Antibody (P004) (abinScience Cat# HT334013, RRID:AB_3729487)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

E3 SUMO-protein ligase CBX4 is a ~61 kDa protein. E3 SUMO-protein ligase that catalyzes sumoylation of target proteins by promoting the transfer of SUMO from the E2 enzyme to the substrate. Also acts as a histone reader, which specifically recognizes and binds histone H3 trimethylated at 'Lys-9' and 'Lys-27' (H3K9me3 and H3K27me3, respectively) via its chromo domain. Catalyzes sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Acts as a regulator of brown adipocyte differentiation by mediating sumoylation of PRDM16, thereby preventing PRDM16 ubiquitination and degradation. Monosumoylates ZNF131.

1. Kagey, MH. et al. (2003) Cell 113, 127-37. PMID: 12679040

2. Oh, Y. et al. (2012) The Journal of biological chemistry 287, 17517-17529. PMID: 22467880

3. Pelisch, F. et al. (2012) The Journal of biological chemistry 287, 30789-99. PMID: 22825850

4. Levine, SS. et al. (2002) Molecular and cellular biology 22, 6070-8. PMID: 12167701

5. Maertens, GN. et al. (2009) PloS one 4, e6380. PMID: 19636380

6. Vandamme, J. et al. (2011) Molecular & cellular proteomics : MCP 10, M110.002642. PMID: 21282530

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CBX4 Antibody (P004)

References

Datasheet

Anti-Human CBX4 Recombinant Antibody (P004).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CBX4 Recombinant Antibody (P004) (HT334013)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us