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Anti-Human CD13/ANPEP Recombinant Antibody (CD13-1), FITC (HB650117)

Anti-Human CD13/ANPEP Recombinant Antibody (CD13-1), FITC
Anti-Human CD13/ANPEP Recombinant Antibody (CD13-1), FITC
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Overview
Catalog No.HB650117
Species reactivityHuman
ApplicationsELISA, FCM, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDCD13-1
ConjugationFITC
Clonality Monoclonal
Target Aminopeptidase M, APN, AP-M, gp150, hAPN, AP-N, PEPN, Microsomal aminopeptidase, CD13, Aminopeptidase N, Alanyl aminopeptidase, Myeloid plasma membrane glycoprotein CD13, ANPEP
Endotoxin level Please contact with the lab for this information.
Associated products Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), FITC
Accession P15144
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Store at 4°C for 12 months. Protect from light. Do not freeze.
Background

Aminopeptidase N (CD13/ANPEP) is a ~109 kDa protein. Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD13/ANPEP Recombinant Antibody (CD13-1), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD13 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD13 monoclonal antibody (Catalog: HB650117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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