





| Catalog No. | HY043107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM |
| Host species | Mouse |
| Isotype | IgG1, kappa |
| Clone ID | 2113 |
| Clonality | Monoclonal |
| Target | T-cell surface glycoprotein CD1a, CD1A, T-cell surface antigen T6/Leu-6, CD1a, hTa1 thymocyte antigen |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P06126 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Background | T-cell surface glycoprotein CD1a is a ~37 kDa protein. Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. |
| References | 1. Sousa SR, et al. Discovery and mode of action of a novel analgesic β-toxin from the African spider Ceratogyrus darlingi. PLoS One. 2017 Sep 7;12(9):e0182848. [HY043107] |
| Note | For research use only. |

SEC-HPLC detection for Anti-Human CD1a Antibody (2113).

Flow-cytometry using anti-human CD1a antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD1a antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.




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