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Anti-Human CD1a Recombinant Antibody (2113) (HY043107)

Anti-Human CD1a Recombinant Antibody (2113)
Anti-Human CD1a Recombinant Antibody (2113)
Anti-Human CD1a Recombinant Antibody (2113)
Anti-Human CD1a Recombinant Antibody (2113)
Anti-Human CD1a Recombinant Antibody (2113)
Anti-Human CD1a Recombinant Antibody (2113)
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Overview
Catalog No.HY043107
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone ID2113
Clonality Monoclonal
Target T-cell surface glycoprotein CD1a, CD1A, T-cell surface antigen T6/Leu-6, CD1a, hTa1 thymocyte antigen
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P06126
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

T-cell surface glycoprotein CD1a is a ~37 kDa protein. Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

References 1. Sousa SR, et al. Discovery and mode of action of a novel analgesic β-toxin from the African spider Ceratogyrus darlingi. PLoS One. 2017 Sep 7;12(9):e0182848. [HY043107]
Note For research use only.
Images
  • Anti-Human CD1a Recombinant Antibody (2113)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD1a Antibody (2113).

  • Anti-Human CD1a Recombinant Antibody (2113)

    Flow Cytometry

    Flow-cytometry using anti-human CD1a antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD1a Recombinant Antibody (2113)

    Flow Cytometry

    Flow-cytometry using anti-human CD1a antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD1a antibody monoclonal antibody (Catalog # HY043107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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