Anti-Human CD21/CR2 Recombinant Antibody (HB5), APC (HB939137)

Name: Anti-Human CD21/CR2 Recombinant Antibody (HB5), APC
Brand: abinScience
SKU: HB939137
Price: 154 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HB939137
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	HB5
Conjugation	APC
Clonality	Monoclonal
Target	Cr2, Complement C3d receptor, C3DR, Epstein-Barr virus receptor, Complement receptor type 2, CR2, EBV receptor, CD21
Endotoxin level	Please contact with the lab for this information.
Associated products	Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173), APC
Accession	P20023
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Complement receptor type 2 (CD21/CR2) is a ~112 kDa protein. Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. 1. Delcayre, AX. et al. (1991) The EMBO journal 10, 919-26. PMID: 1849076 2. van den Elsen, JM. et al. (2011) Science (New York, N.Y.) 332, 608-11. PMID: 21527715 3. Barel, M. et al. (1995) Molecular immunology 32, 389-97. PMID: 7753047 4. Bradbury, LE. et al. (1992) Journal of immunology (Baltimore, Md. : 1950) 149, 2841-50. PMID: 1383329 5. Franco, A. et al. (2018) International immunology 30, 569-578. PMID: 30107486 6. Barel, M. et al. (2003) European journal of immunology 33, 2557-66. PMID: 12938232 7. Aubry, JP. et al. (1992) Nature 358, 505-7. PMID: 1386409
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow Cytometry

Flow-cytometry using APC anti-human CD21 antibody. Raji cells were stained with an irrelevant antibody (Green Histogram) or an APC anti-human CD21 antibody monoclonal antibody (Catalog # HB939137, Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet