Anti-Human CD213a2/IL13RA2 Recombinant Antibody (47#) (HC579107)

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Overview

Catalog No.	HC579107
Description	Anti-Human CD213a2/IL13RA2 Antibody (47#) [47#] (HC579107) is a mouse monoclonal antibody detecting CD213a2 in ELISA, FCM, IHC, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, IHC, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	47#
Clonality	Monoclonal
Target	IL13R, IL-13RA2, IL-13 receptor subunit alpha-2, IL-13R-alpha-2, Interleukin-13-binding protein, CD213a2, IL-13R subunit alpha-2, IL13RA2, Interleukin-13 receptor subunit alpha-2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q14627
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Interleukin-13 receptor subunit alpha-2 (CD213a2/IL13RA2) is a ~44 kDa protein. Cell surface receptor that plays a role in the regulation of IL-13-mediated responses. Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation. Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1. 1. Rahaman, SO. et al. (2002) Cancer research 62, 1103-9. PMID: 11861389 2. Andrews, AL. et al. (2006) The Journal of allergy and clinical immunology 118, 858-65. PMID: 17030238 3. Fichtner-Feigl, S. et al. (2006) Nature medicine 12, 99-106. PMID: 16327802
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD213a2 antibody.A375 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD213a2 antibody monoclonal antibody (Catalog # HC579107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD213a2/IL13RA2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD213a2/IL13RA2 (Catalog No: HC579011)
Lane 2: negative control

References

Datasheet

Anti-Human CD213a2/IL13RA2 Recombinant Antibody (47#).pdf

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Anti-Human CD213a2/IL13RA2 Recombinant Antibody (47#) (HC579107)

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