Anti-Human CD230/PRNP Recombinant Antibody (ICSM 18) (HY392107)

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Overview

Catalog No.	HY392107
Description	Anti-Human CD230/PRNP Antibody (ICSM 18) [ICSM 18] (HY392107) is a mouse monoclonal antibody detecting CD230 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	ICSM 18
Clonality	Monoclonal
Target	PRNP, PrP27-30, PRIP, PrP, CD230, ALTPRP, ASCR, PrP33-35C, PRP, Major prion protein
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P04156
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Major prion protein (CD230/PRNP) is a ~27 kDa protein. Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis.
Note	For research use only.

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Flow-cytometry

Flow-cytometry using anti-human CD230 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD230 antibody monoclonal antibody (Catalog # HY392107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

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Formula

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Working Solution

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Overview