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Anti-Human CD235a/GYPA Recombinant Antibody (NNA7) (HF002107)

Anti-Human CD235a/GYPA Recombinant Antibody (NNA7)
Anti-Human CD235a/GYPA Recombinant Antibody (NNA7)
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Overview
Catalog No.HF002107
Description
Anti-Human CD235a/GYPA Antibody (NNA7) [NNA7] (HF002107) is a mouse monoclonal antibody detecting CD235a in FCM. Suitable for Human.
Highlights
  • Flow Cytometry — Validated for flow cytometry applications.
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone IDNNA7
Clonality Monoclonal
Target PAS-2, Glycophorin-A, Sialoglycoprotein alpha, CD235a, MN sialoglycoprotein, GYPA, GPA
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P02724
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 0.09% Sodium azide.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Glycophorin-A (CD235a/GYPA) is a ~16 kDa protein. Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.

1. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865
Note For research use only.
Images
  • Anti-Human CD235a/GYPA Recombinant Antibody (NNA7)

    SDS-PAGE

    SDS-PAGE for Anti-Human CD235a/GYPA Antibody (NNA7)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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